FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14658752
Sequences flagged as poor quality0
Sequence length35-101
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG160499910.949083523617837No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC2536791.7305634204057754No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG2296291.5664976118021507No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC2007021.3691615766471799No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG1875871.2796928415188413No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG1490581.0168532764590055No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG1377400.9396434293997197No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG1183930.8076608431604546No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG1106220.7546481446715245No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT959460.6545304811760237No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA885260.603912256650498No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG858230.5854727605733422No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG814840.5558726963932537No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG787850.5374604877686723No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC718400.4900826482363574No Hit
TGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG629290.42929302576372125No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN594380.4054779015294071No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG520300.35494153936160455No Hit
TGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCG514790.35118269276947994No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC512360.3495249800255847No Hit
GAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG493980.3369863955676445No Hit
AGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC483280.3296870020039905No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC480640.32788603013407963No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC459730.31362151430080815No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG434990.29674422488353713No Hit
AGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG392390.26768308789179324No Hit
CGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGGTCTCC375060.2558607990639312No Hit
TAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG361070.24631701252603222No Hit
CGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG358430.24451604065612137No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCG338280.2307699864217636No Hit
GTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG329110.2245143379190807No Hit
AAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG290980.19850257375252683No Hit
GGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG290680.1982979178582188No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC288170.1965856302091747No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG285090.19448449636094534No Hit
GGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA283940.19369998209943112No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG276200.18841986002628328No Hit
GAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGG272850.18613453587317663No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA272580.1859503455682994No Hit
CAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG265500.18112046646262928No Hit
TGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG263600.17982431246534494No Hit
AGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCC259530.177047814165899No Hit
GGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG252240.17207467593421324No Hit
GCGCTAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG249060.16990532345454784No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG246280.16800884550062653No Hit
CAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCC244770.16697874416594263No Hit
TGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG242080.16514366298031374No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234160.15974074737058105No Hit
TGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG233470.15927003881367255No Hit
ACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCAC233430.15924275136109814No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG232900.15888119261448724No Hit
ATGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG230250.15707339888143274No Hit
GCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGGTCTCCC222550.15182056426085933No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA221880.15136349943023797No Hit
AGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG221800.15130892452508918No Hit
TGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA221160.15087232528389866No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG221140.15085868155761145No Hit
GGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGG218380.14897584732997735No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG217400.14830730474190437No Hit
TGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG210460.14357293172024468No Hit
GCCGGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC204980.13983455071755085No Hit
TCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG204270.13935019843435512No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG204040.1391932955820523No Hit
TGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG196460.13402232331920208No Hit
CCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG195850.13360618966744237No Hit
TTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC194010.13235096684901962No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG192640.13141637159834615No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC192300.1311844282514637No Hit
TGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCC188990.12892639155093147No Hit
TTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG188590.1286535170251874No Hit
ACGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG184700.1259998122623263No Hit
GGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC184280.12571329401029502No Hit
GTTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG183430.12513343564308885No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA183010.12484691739105758No Hit
AGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA179440.12241151224879172No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA176640.1205013905685832No Hit
TGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG175130.11947128923389932No Hit
GTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG175020.1193962487393197No Hit
GTGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG174530.11906197744528318No Hit
GGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGG172970.1179977667948813No Hit
GTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTT167530.1142866732447619No Hit
GTGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG167530.1142866732447619No Hit
GATGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG164090.11193995232336286No Hit
TAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG163950.11184444623935244No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC163330.11142149072444912No Hit
CCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCC161160.10994114642228753No Hit
AGTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG160050.10918391961334771No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC156760.1069395266391027No Hit
GTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC155140.10583438480983921No Hit
CCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG154630.10548646978951551No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT149420.10193227909169894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)