FastQCFastQC Report
Thu 29 Sep 2022
EGAF00007040316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007040316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33764015
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCGAGATCTCGTATG10936153.238995717778232TruSeq Adapter, Index 15 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT678890.20106909678840032Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT506570.15003251242484048Clontech SMART CDS Primer II A (100% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCGAGAGCTCGTATG446510.1322443435711067TruSeq Adapter, Index 15 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)