FastQCFastQC Report
Thu 29 Sep 2022
EGAF00007040317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007040317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33764015
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT9326232.7621803864261993Illumina Single End PCR Primer 1 (100% over 51bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6317921.8711992634762187Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG1553990.46025035825863714Illumina Single End PCR Primer 1 (98% over 50bp)
AAGCAGTGGTATCAACGCAGAGTAGGGTAAAACTAACCTGTCTCACGACGG441070.1306331607778281Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)