FastQCFastQC Report
Thu 29 Sep 2022
EGAF00007040318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007040318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31621546
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCTTAGATCTCGTATG12697224.015369773508228TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCTTAGAGCTCGTATG462570.14628317034214583TruSeq Adapter, Index 9 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT413710.130831680399181Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCTTAGACCTCGTATG333680.10552298739599893TruSeq Adapter, Index 9 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)