FastQCFastQC Report
Sat 22 Oct 2022
EGAF00007075917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007075917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20732352
Sequences flagged as poor quality0
Sequence length98
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT276809013.351548343381397Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6626993.1964487193734703Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2682721.293977644215186Clontech SMART CDS Primer II A (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2142321.0333222202671457Clontech SMART CDS Primer II A (100% over 21bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680080.8103663298790219Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1374080.6627709195753574Clontech SMART CDS Primer II A (100% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1203050.580276661326221No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA448870.2165070321013265Clontech SMART CDS Primer II A (100% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA397070.19152192669698065Clontech SMART CDS Primer II A (100% over 21bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTA292760.14120925594934913Clontech SMART CDS Primer II A (100% over 24bp)
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT283660.13681998067561268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273910.13211718573946651No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT265620.12811860419888685No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG261970.1263580707099706No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC250910.12102341306958324No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA249010.12010697097946242No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT240160.11583828019126823No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA237110.11436714946765325No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCG225290.1086659149912176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)