FastQCFastQC Report
Sat 22 Oct 2022
EGAF00007075943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007075943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6335478
Sequences flagged as poor quality0
Sequence length98
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3203895.0570612035903215Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT709451.1198050091879412Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT302640.4776908703652668Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG265170.4185477402020811No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237470.3748257037590534Clontech SMART CDS Primer II A (100% over 21bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT231370.36519738526437945Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200190.31598247204078367Clontech SMART CDS Primer II A (100% over 22bp)
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC75040.11844410161316953No Hit
CTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA64690.10210752842958339No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)