FastQCFastQC Report
Sat 22 Oct 2022
EGAF00007075949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007075949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14782611
Sequences flagged as poor quality0
Sequence length98
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7356704.976590400707967Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1648181.1149451203173784Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT702080.47493639655403236Clontech SMART CDS Primer II A (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT576710.3901272921272162Clontech SMART CDS Primer II A (100% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG564300.3817322934358484No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT541290.3661667076269544Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT477160.32278465556592134Clontech SMART CDS Primer II A (100% over 22bp)
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC169490.11465498212731161No Hit
CTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA148540.10048292551295573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)