FastQCFastQC Report
Sat 22 Oct 2022
EGAF00007076145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007076145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9616499
Sequences flagged as poor quality0
Sequence length98
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT163703417.02318068145174Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4088734.251786434959334Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1783171.8542818961453644Clontech SMART CDS Primer II A (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1262271.312608673905129Clontech SMART CDS Primer II A (100% over 21bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1129571.1746166666268048Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT892540.9281340329781139Clontech SMART CDS Primer II A (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA417690.4343472608898519Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG360400.3747725653587652No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA356910.37114338596614005Clontech SMART CDS Primer II A (100% over 21bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTA281370.2925908898862257Clontech SMART CDS Primer II A (100% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA228730.23785163394703207Clontech SMART CDS Primer II A (100% over 21bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTA163370.1698851109951761Clontech SMART CDS Primer II A (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTAA153060.15916395353444118Clontech SMART CDS Primer II A (100% over 26bp)
CTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA150470.15647066567573084No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC147670.15355900312577372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134080.1394270409636605No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA127400.13248064602304852Clontech SMART CDS Primer II A (100% over 23bp)
GTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAA124080.12902824614238506No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTAA122670.12756201607258524Clontech SMART CDS Primer II A (100% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA116540.12118755484714344Clontech SMART CDS Primer II A (100% over 21bp)
GTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA104470.10863620949786404No Hit
GTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGC101240.10527739877059208No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT99890.1038735614697199No Hit
CTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGG99610.10358239521472419No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG99240.103197639806337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)