FastQCFastQC Report
Sat 22 Oct 2022
EGAF00007076279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007076279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8489742
Sequences flagged as poor quality0
Sequence length98
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5187236.109997217818869Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1227121.4454149490055175Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT481400.5670372550779518Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG424870.500451014883609No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335270.3949118830701805Clontech SMART CDS Primer II A (100% over 21bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT307520.36222537740251703Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277040.3263232263124133Clontech SMART CDS Primer II A (100% over 22bp)
CTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA146940.1730794646056382No Hit
GTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAA121370.1429607637075426No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC116050.13669437775612028No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA113590.13379676319963552No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT112600.1326306500244648No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGT104930.12359621764713227No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTC98220.11569256168208646No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT92170.10856631450048776No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA91450.1077182321912727Clontech SMART CDS Primer II A (100% over 26bp)
CTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGG90250.1063047616759143No Hit
GGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT88630.10439657648018044No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG88080.10374873582730783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)