FastQCFastQC Report
Sat 22 Oct 2022
EGAF00007076285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007076285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15382115
Sequences flagged as poor quality0
Sequence length98
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9249836.013366822442817Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2205581.4338600381026927Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT866160.5630955170989165Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG648310.4214699994116544No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT631920.41081476767011554Clontech SMART CDS Primer II A (100% over 21bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT558840.36330504615262593Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT502050.3263855458108329Clontech SMART CDS Primer II A (100% over 22bp)
CTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA261440.169963623337883No Hit
GTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAA223710.14543513684561585No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC211790.137685877397224No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT203490.13229000043232028No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA200520.13035918662680654No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGT189950.12348756981728455No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTC179270.11654444138533616No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT169630.11027742283814677No Hit
CTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGG165630.10767700020445822No Hit
GGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT160590.10440046768601066No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG158120.102794706709708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)