FastQCFastQC Report
Sat 22 Oct 2022
EGAF00007076393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007076393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8836070
Sequences flagged as poor quality0
Sequence length98
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT317296135.90918813454398Clontech SMART CDS Primer II A (100% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6706227.589595827104131Clontech SMART CDS Primer II A (100% over 21bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3228673.653966073152431Clontech SMART CDS Primer II A (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2377782.690992715087137Clontech SMART CDS Primer II A (100% over 24bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2011332.2762721436113567Clontech SMART CDS Primer II A (100% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1897802.1477874213309764Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1889102.1379414151313876No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA1208071.367202840176685Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA948231.0731354550156347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA905391.0246523624190393No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA741010.8386194314893385Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA722570.8177504252456126No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA622270.7042384227377103Clontech SMART CDS Primer II A (100% over 21bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT583120.659931394839561Clontech SMART CDS Primer II A (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA405130.4584956886941819No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383280.43376750071015735No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTA353370.3999176104308816Clontech SMART CDS Primer II A (100% over 22bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA346800.39248217816291636Clontech SMART CDS Primer II A (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG282890.3201536429657076No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA281160.31819575897429514No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTAA272830.30876849096940157Clontech SMART CDS Primer II A (100% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA272400.30828184928367475Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA260490.29480300631389295No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA232590.2632278829841773No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAA192500.21785703372653228Clontech SMART CDS Primer II A (100% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA190880.21602363946867784No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG185000.2093690973475765Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA184980.2093464628505659No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172990.1957770818927419No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTAA168540.1907409063078948Clontech SMART CDS Primer II A (100% over 23bp)
TGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166300.1882058426427133No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTA154920.17532681384371107Clontech SMART CDS Primer II A (100% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT139780.15819249960672563Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA131300.14859547287425293No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA129310.14634334042170333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT106490.12051737933266712No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA102750.11628472839169449No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA102610.11612628691262064No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA100550.11379493372053412Clontech SMART CDS Primer II A (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT99980.11314985055573348Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAG97660.11052424890250982Clontech SMART CDS Primer II A (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG96880.10964150351909842Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG89180.10092722217003712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)