FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007133877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007133877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53728734
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGCTTCTGTGTAGATCT5632601.0483403535992493Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGCTTCTGTGTAGATC2105920.391954145057652Illumina Single End PCR Primer 1 (97% over 34bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1765000.32850206371882873No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1580100.2940884480918534No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA991860.18460513139952264No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG853720.1588944939592286No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT658060.12247822552453962No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT602840.11220067087380098No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT543860.10122330446125903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)