FastQCFastQC Report
Sun 12 May 2024
EGAF00007133881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007133881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56995423
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATCACGTGTGTAGATCT2632220.46183006660026016Illumina Single End Sequencing Primer (100% over 32bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1733140.30408406653986936No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1217580.21362768024372764No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG990600.17380342979470476No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT912100.1600303940195338No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC799040.140193713449587No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATCACGTGTGTAGATC715860.12559955910845683Illumina Single End Sequencing Primer (100% over 33bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT698280.12251510090555869No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA665450.11675498925589164No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT625390.10972635469342863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)