FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007133885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007133885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55985795
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2166600.38699102156180865No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1436740.2566258101720267No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1024120.18292497230770768No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG987170.17632508388958307No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCAACCATGTGTAGATCT861490.15387653243112828Illumina Single End PCR Primer 1 (96% over 32bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT764430.13653999197475003No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA761920.13609166396583275No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT707770.12641956767783685No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA615610.10995824923089866No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC596220.10649487070782866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)