FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007133887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007133887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57424183
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1164560.20279957661739825No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCTGGTAGTGTAGATCT1044490.18189026738090466Illumina Single End PCR Primer 1 (96% over 32bp)
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA903520.15734137654165667No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC725250.12629696446878488No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT690190.12019152279449932No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG678150.11809484516305613No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCTGGTAGTGTAGATC605510.10544512231022947Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)