FastQCFastQC Report
Fri 10 May 2024
EGAF00007133895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007133895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52456329
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACTTGGGTGTAGATCT5917961.1281689193309734Illumina Single End Sequencing Primer (100% over 32bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACTTGGGTGTAGATC2298790.4382292935519754Illumina Single End Sequencing Primer (100% over 33bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1288540.2456405212800919No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1255360.23931525974682674No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA858920.16374001314502964No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG644460.12285648124557096No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC582630.1110695336686637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)