FastQCFastQC Report
Mon 3 Apr 2023
EGAF00007133899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007133899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51235363
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAATCCGGTGTAGATCT2957120.5771638623893423Illumina Single End PCR Primer 1 (96% over 32bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT920060.17957518911303508No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC900090.17567749056447596No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAATCCGGTGTAGATC887810.17328070848253774Illumina Single End PCR Primer 1 (96% over 33bp)
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA671750.1311106159236151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)