FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007134348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007134348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67283622
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGC52474267.798964806026644TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG21992243.2685874134421598TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTATGC8868321.3180503273144244TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAGCTCGTATGC1026350.15254083675816382TruSeq Adapter, Index 7 (97% over 35bp)
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATC829800.12332867573627354No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACCTCGTATGC716740.10652518082335104TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)