FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007134349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007134349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67283622
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTCG34751475.164922601818315Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC21954713.2630095329885775Illumina Single End PCR Primer 1 (97% over 34bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGGCTATAGTGTAGATCTCG2226210.3308695242357791Illumina Single End PCR Primer 1 (96% over 33bp)
ATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTCG1365510.20294834900534933Illumina Single End PCR Primer 1 (96% over 33bp)
ATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTCG1141270.16962077338821027Illumina Single End PCR Primer 1 (96% over 33bp)
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCC792260.11774930903690053No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGGCTATAGTGTAGATCTCG774660.11513351644475978Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)