FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007134694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007134694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69630013
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATGC7811361.12183807864577TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG4340020.6232973128986778TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAACTCGTATGC1330890.19113740507272345TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph