FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007231936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007231936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56124793
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1476340.2630459590291941No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTAT1369590.2440258443358535TruSeq Adapter, Index 4 (97% over 38bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1128680.2011018552888026No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA899310.16023399854677414No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT824500.14690477343943167No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC809790.1442838283608458No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG694390.12372250531062093No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT683490.1217804046065702No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCGCGTAT672350.1197955420521551TruSeq Adapter, Index 4 (97% over 38bp)
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC579960.1033340114056189No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC576080.10264269482472746No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)