FastQCFastQC Report
Thu 5 Jan 2023
EGAF00007231940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007231940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60399059
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1164380.19278114912353186No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGTT978020.16192636378656164TruSeq Adapter, Index 19 (97% over 37bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT919160.15218117884916055No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT686370.11363918765688054No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG646600.10705464798714827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)