FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007249825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007249825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471397326
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG27186350.5767183753604915TruSeq Adapter, Index 6 (97% over 35bp)
CTTGATCTCATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT21458090.4552017760066802No Hit
CTTGATCTCATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA13721990.29109180818730396No Hit
CTTGATCTCATGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC6281480.13325234687478904No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCCCGTATG4886770.10366562834512134TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)