FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007256294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007256294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519854869
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTGGCCTTCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT21201820.4078411353688792No Hit
AGCTGGCCTTCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA17174510.3303712444405325No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC7160400.13773844253442974Illumina Single End PCR Primer 1 (96% over 31bp)
AGCTGGCCTTCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC6549880.1259943955627354No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5864190.11280436809758917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)