FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007256356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007256356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512644746
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC74374401.450798054214331Illumina Single End PCR Primer 1 (96% over 31bp)
AGCGACTGTATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT24139080.47087344966176636No Hit
AGCGACTGTATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA19409140.37860799611120954No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC8243150.16079653725740126No Hit
AGCGACTGTATGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC5593780.10911610903352553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)