FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007257879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007257879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294749398
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT14867110.5043983160230238Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT10961100.37187862212359807Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA6014720.20406216402179045No Hit
AAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACC5844210.1982772497469189No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4644380.15757046601330124Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC4323160.14667239456075157No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC4206450.14271275967118344No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA3375090.11450710409932711No Hit
CCGAAACCAAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCT3309870.11229437693372321No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT3181700.10794593717880978No Hit
TCCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTG3176270.10776171288397339No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT3139000.10649724889344812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)