FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007257885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007257885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262013508
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT6198990.23659047380106832Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT4146710.15826321442938734Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAATAATTCAAGCACTGCTCATTACAATTTTACTGGGTCTCTATTTTACC3773560.14402158227659012No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT3323300.1268369720846606No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC3094110.11808971314562912No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2964780.11315370809050045No Hit
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC2838710.10834212410147953No Hit
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC2795380.1066883925694396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)