FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007257897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007257897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346778359
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11659620.3362268635684962Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA10225960.29488460668331384No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT8216690.2369435631362452Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT5676360.16368841517010582No Hit
AAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAG4347530.12536912662419053No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT3929530.11331531792616852Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC3612650.10417749280600293No Hit
CTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG3592180.10358720222215481No Hit
GAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTA3584540.1033668885894924No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT3570140.10295163776353183No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC3488660.10060201017330496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)