FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007257899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007257899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242569142
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT6809110.2807080053076166Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC5836090.2405949063380865No Hit
CTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCAC5304670.2186869259734612No Hit
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT5147930.21222526317877646No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT4394060.18114670166908536No Hit
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC4378560.18050770860211066No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA3952710.16295188940397043No Hit
CTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAG3920750.16163432692522778No Hit
CCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGAC3652880.15059128996713028No Hit
CCGAAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCT3436860.14168578788146105No Hit
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC3433100.14153078053102072No Hit
CAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTC3402020.14024949636833856No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC3195780.13174717829525076No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA3184160.13126813962181552No Hit
ATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCT3160660.1302993436815636No Hit
CCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAAC3130780.129067529949873No Hit
CCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTT3050880.12577362375301637No Hit
GTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAG3029160.1248782089520686No Hit
ACCGAAACCAAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTC3015730.1243245523785544No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG2952660.12172446897635479No Hit
GGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTG2926990.12066621400672638No Hit
CTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGG2802940.11555220820297085No Hit
CACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCC2786160.1148604466762718No Hit
CAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCA2713560.11186748560128065No Hit
CACAAACATTATTATAATAAACACCCTCACCACTACAATCTTCCTAGGAA2686160.11073791076030602No Hit
CATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA2661440.10971881988187929No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT2647950.10916268978681551No Hit
TCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACC2637780.10874342788416179No Hit
CCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCA2622960.10813246806141566No Hit
CCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGC2622860.1081283455254997No Hit
GGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAG2597430.1070799846420696No Hit
CTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAA2586620.1066343385095537No Hit
TTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTAC2557650.10544003985469841No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT2537720.10461841844664643Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTAC2486460.10250520653612238No Hit
ATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAG2485880.10248129582780979No Hit
CCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATA2463330.1015516639787595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)