FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007257915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007257915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298759267
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAG6873880.23008089653667546No Hit
GCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCAT6052470.20258685398367912No Hit
GTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGG5996050.20069837699795934No Hit
GGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCAT5945000.19898964339071032No Hit
ACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTC5555930.1859667837516819No Hit
CACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGT5221220.17476344926231194No Hit
GGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAG5002270.16743480629841015No Hit
GCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAA4996030.16722594248432132No Hit
CAAAGAGCTTCAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTG4775370.1598400628021356No Hit
CTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAGGGAGAAGT4642570.15539501239973252No Hit
GCTTCAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTC4622750.15473160201587988No Hit
GCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAGG4588670.15359088426201017No Hit
GTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCC4441550.14866651818368534No Hit
GCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCA4268740.142882262460498No Hit
GAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTC4181920.13997624381639684No Hit
CGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4103950.13736645029323893No Hit
CTTCAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCA4055840.13575612367532017No Hit
CTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCA4016220.13442997234291648No Hit
CCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAG3987130.13345627869678767No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3934340.1316893042183023No Hit
GCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAGGGAGAAG3760650.12587559334184603No Hit
GCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGTGG3711050.12421539379396054No Hit
GGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCT3641400.12188408535625442No Hit
GCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCC3517550.11773860725130243No Hit
GAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGC3442980.11524261772941088No Hit
GCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAG3393260.11357840156971599No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3190670.1067973566824958No Hit
AAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAG3181440.10648841229082277No Hit
GTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCA3127750.10469131322376687No Hit
GCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAAC3095830.10362289448246638No Hit
GGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGC3037040.101655089413511No Hit
AAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCA2993930.1002121216209839No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)