FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007257917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007257917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269118608
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT18091530.6722511733562474Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT8551550.3177613790273469Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3884970.14435902551933533Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAATAATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACC3665360.13619868307285538No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2707290.10059839489062757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)