FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007257919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007257919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290706403
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4636520.15949149905721202Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT4540350.15618335038874256Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCAT4330610.14896851102381808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA4308770.14821723758179486Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA3719320.12794076640960675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT3527850.12135439617406707Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3253220.11190740783236204No Hit
CTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTC3203750.11020569092865837No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA3190850.10976194425273804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)