FastQCFastQC Report
Sat 17 Dec 2022
EGAF00007260364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007260364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438389171
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG6073888113.855013996228479TruSeq Adapter, Index 1 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCCCGTATG8031080.18319521856984922TruSeq Adapter, Index 1 (97% over 35bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG4949220.11289558062555335TruSeq Adapter, Index 13 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCGCGTATG4872560.11114690604435574TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)