FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45101717
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGAC1559380.34574736921878163No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGG1456700.3229810519187108No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT1199010.2658457548301321No Hit
GTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGA1142640.2533473393041777No Hit
GTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCCC1120650.24847169343907685No Hit
GTTACTCCCGCCGTTTACCCGCGCTTCATTGAATTTCTTCACTTTGACATT1112560.2466779701535531No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAA1106770.24539420528047745No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCC1106670.2453720331755884No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT1087880.24120589466693695No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC1053370.2335543012697277No Hit
GTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTCC1048180.2324035690259863No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACA1036260.22976065412321217No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAA986050.218628040258423No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT951590.21098753291365824No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTG939050.2082071509605721No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGAT928450.20585690784233335No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCAC925780.20526491264179586No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTT917270.2033780665157382No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCA915070.2028902802081792No Hit
GTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGA899340.19940260810913255No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAAC883880.19597480069328627No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGA868040.1924627392788616No Hit
GGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATC856140.18982425879706533No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATT838330.1858754069163265No Hit
CGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAG834660.18506169066689857No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC833150.18472689188307398No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCT830690.18418145810280348No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAA799450.177254892535466No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACA776250.1721109642012077No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGG758590.16819537047780242No Hit
AGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAA756020.167625547382154No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCG752390.16682069997468169No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTC752250.16678965902783702No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGG740680.16422434649217457No Hit
GGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCT727400.16127989096290946No Hit
TGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTC724780.16069898181481648No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC709980.1574175102912379No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGG701270.15548631995540213No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCC700610.15533998406313446No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGC698470.1548655010185089No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGC675060.14967501126398358No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCT673180.14925817569206956No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC673070.1492337863766916No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCA666940.1478746363469932No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCACG658070.14590797064333494No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCC653800.1449612217645727No Hit
AGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGT646660.14337813347549494No Hit
ACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC646440.14332935484473905No Hit
GTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCG624490.1384625778215938No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTAC620890.1376643820455882No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAA620630.1376067345728767No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTC599710.13296833023008858No Hit
CCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACT581270.12887979408854877No Hit
CTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCC564710.125208093518923No Hit
CCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCT562600.12474026210576418No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACACGCGC559850.12413052922131546No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG557870.12369152154451236No Hit
TGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGCA549980.12194214246876676No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATT549300.12179137215552127No Hit
GTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACT544310.12068498412155795No Hit
CCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGG542590.12030362391746638No Hit
GTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTT540170.11976705897915149No Hit
ATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCG540030.11973601803230685No Hit
GACCGATTTGCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTG534460.11850103178998707No Hit
GTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTCTCGTGCCG533150.11821057721594058No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTAC532920.11815958137469577No Hit
GGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCAT532670.11810415111247316No Hit
GTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAA525000.11640355066748345No Hit
CGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTC521320.1155876172075666No Hit
GCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCG518990.11507100716365189No Hit
AGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGA517860.11482046237840568No Hit
GTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCC514200.11400896333946665No Hit
CGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCA512520.1136364719773307No Hit
GTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACC511320.11337040671866216No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC508810.11281388688594716No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAAC504980.11196469526869675No Hit
ACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGG504300.11181392495545124No Hit
GGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAG493280.10937055899667854No Hit
GTTCAACTGCTGTTCACATGGAAGCCTTCTCCACTTCGGCCTTCAAAGTTC492590.10921757147294414No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTA491760.10903354300236508No Hit
GCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGG491410.10895594063525342No Hit
GTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA490940.10885173174227492No Hit
TGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCCCT489120.1084481994332943No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG489000.10842159290742745No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACC487310.1080468843348026No Hit
GTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCC485970.1077497781292894No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC483450.10719104108608549No Hit
GCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCT482560.10699370935257299No Hit
TGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCC481330.10672099246243774No Hit
GTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCT481020.1066522589372817No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTT479530.10632189457443494No Hit
CACACCCCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACACGCGCGC477390.10584741152980938No Hit
AGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGA476140.10557026021869632No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCT474500.105206637698516No Hit
GGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCT471900.10463016297140085No Hit
GGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTC471330.1045037819735333No Hit
AGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGT464870.10307146399770102No Hit
CCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACG463920.1028608290012551No Hit
GCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGC462900.10263467353138685No Hit
GGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGGC461000.102213403538495No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCG460600.10212471511893881No Hit
CACACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACT458080.1015659780757349No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCC457690.10147950686666762No Hit
CTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGC457540.10144624870933405No Hit
GGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGC455110.10090746656053029No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTT452640.10035981556977087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)