FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53461568
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCTCG17265023.2294264171226703No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT5376111.0056027537389103No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT5079590.9501386117219757No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG4959940.927758048548071No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT4264680.797709487308715No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG3723110.6964086799698804No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA3226440.6035064291417715No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG3224420.6031285876239171No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2947180.5512707745496728No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT2921340.5464373959252373No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACATATGCATGTATCGCG2568840.4805021805570686No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2431620.45483514437885547No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC2292680.42884638175969697No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA2269430.4244974632992433No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2157060.4034786259916656No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT1498630.2803191256941809No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1410730.2638774081598205No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA1320380.24697741749736932No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT1089200.20373513923123243No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1071410.20040751517052402No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT1063350.19889989010423337No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT1017290.190284355296126No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT956670.1789453687553646No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC955790.17878076452976463No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC954440.17852824668367376No Hit
GGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATAT945020.17676623326872867No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT942530.17630047813038333No Hit
GCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCC897990.1679692597119486No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG866590.16209588166213157No Hit
GGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGAAGAGGGCACAGACGGG852770.15951084711918664No Hit
CCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG848670.1587439410680959No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC841120.1573317116325507No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA800820.14979358630109765No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG799490.14954480946013404No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA795250.14875171637315238No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT736830.1378242403963909No Hit
GCCGGGGAGAGCGAGCGGGGCCGTGCCCGGCGGCGCGGAGCGGCGCGGCG724280.13547675967902775No Hit
GGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGC723350.13530280294060962No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG689640.12899733879859265No Hit
CGGCGGGCGGCGGGCGGGGAAGAGGGCACAGACGGGCGAGGGCCGGGGAC683840.12791244731168377No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG673640.12600453469677508No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG636560.11906871119081282No Hit
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC631710.11816151744744935No Hit
GCGAGGAGGACGGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGG623340.11659590680168602No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA623010.11653418021708604No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG618740.11573547562241347No Hit
GCGGGAACGACACACCACCGTTCGGCCTCGGGCACCTGAGGGACAACCCG618290.11565130300704984No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA593300.1109769171005235No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC588680.11011274491612368No Hit
GGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGG569820.10658497708110619No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT568710.10637735129654258No Hit
GCGGAGGTGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATAT568370.10631375420937896No Hit
GACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT547090.10233332475396158No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT540460.10109318155427091No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)