FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36476539
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAATTGGAATCTCG10910822.9911883909819403No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT1831990.5022378904972317No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGAATTGGAATCGCG1501680.41168379489073786No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT1450590.3976775318513634No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT1435980.39367221764104315No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1350540.37024894275194253No Hit
GGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATAT1300570.35654972638714433No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1259450.3452767270491315No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1235120.3386066863415962No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1134550.3110355398575506No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1123660.30805005924493No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1100790.3017802758096101No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT1089340.2986412718597014No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1005450.2756429276363089No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG971190.26625058918007544No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT970730.26612448072444594No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG948220.259953390863097No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT920380.25232108780934503No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA908310.24901211159315306No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA892880.2447819953532324No Hit
GCGGAGGTGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATAT885910.24287117810162856No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT840010.23028774742033503No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG743400.2038022302499697No Hit
GGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAG733980.20121974839773038No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA688370.1887158208732468No Hit
GGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATATC684440.18763841602406414No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG683710.18743828738795643No Hit
GTGGAAGTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG683360.18734233530215133No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT671630.18412656968359856No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA655370.17966890992591156No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA649410.1780349829790595No Hit
GCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGA619830.1699256609844481No Hit
CTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAA614800.16854669243702095No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA602690.16522675026816552No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC596120.16342559254319605No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC591430.1621398345934081No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT590470.16187665172948562No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG590380.16185197833599288No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA584570.16025917371162873No Hit
GTGGGGATAGCGATGATTATGGTAGCGGAGGTGAAATATGCTCGTGTGTC581360.15937915601038793No Hit
GTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATATCGGGGGCACCG569170.1560372819362056No Hit
GGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTT566110.15519838655745272No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA558220.15303535239458985No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC557600.15286538012830658No Hit
GTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTC537030.1472261389711343No Hit
GGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATATCGGG531710.14576766726689722No Hit
GAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGG531300.145655266252097No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT524660.14383491810996651No Hit
CCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAAT510300.13989814110379278No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC504170.13821760885812112No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG503350.13799280682852066No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCTACAACGT502240.1376885016421103No Hit
GTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGA491550.13475785079280683No Hit
GTGGAGTGTGGCGAGTCAGCTAAATACTTTGACGCCGGTGGGGATAGCGA485990.13323358337258914No Hit
GTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGA485960.1332253589080916No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT480040.13160239791390296No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG476460.13062094515052539No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA456630.12518457411762668No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC453780.12440324999035682No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC449100.12312023352873475No Hit
GTTGTGATGAAATTGATGGCCCCTAAGATAGAGGAGACACCTGCTAGGTG442840.1214040619369069No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT442390.12128069496944323No Hit
GTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGT439020.1203568134575487No Hit
GTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCTA437510.11994284874450396No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA433790.11892301514680435No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA432800.1186516078183843No Hit
GCTAGGTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGA427870.11730005415261574No Hit
GGACATAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATG419010.11487109563766452No Hit
GGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTT415290.11385126203996492No Hit
GGGGATAGCGATGATTATGGTAGCGGAGGTGAAATATGCTCGTGTGTCTA413270.11329748143046137No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT410310.11248600093336707No Hit
GGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTG399020.10939086079411209No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA397900.10908381411953585No Hit
CGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCA395150.10832990487392458No Hit
GCGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGT393750.1079460965307043No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG392980.10773500194193315No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT392080.10748826800700581No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC386140.10585982403648547No Hit
GGTGGGGATAGCGATGATTATGGTAGCGGAGGTGAAATATGCTCGTGTGT384230.10533619979680638No Hit
GTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATATCGGGG378390.10373517070794464No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA377100.1033815187345488No Hit
GGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAG375520.10294836360434305No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC375160.10284967003037213No Hit
GTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCC368940.10114446439120774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)