FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36476539
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGATCAGATTAACGTGTAGA7970672.1851497478968605No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1551590.4253665623265409No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCAGATTAACGTGTAGT1462700.4009974740202189No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1316520.36092240001168974No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG1274910.34951506775354974No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT1168790.32042239533745237No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA1117310.30630921425960944No Hit
GGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCC1064910.2919438162705075No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT882020.24180473920510934No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT879860.24121257776128377No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA843630.23128016613637603No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG810630.22223325518904083No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT794310.2177591465023587No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCAGATTAACGTGTTGA782670.21456805427729864No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGG724750.19868935482064237No Hit
GGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGA717090.19658937488559428No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT667120.18289015852079607No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC665750.18251457464207335No Hit
GGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGC657290.1801952756537565No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA653120.17905207508859325No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG651780.1786847156743681No Hit
GAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCAC645740.1770288568221892No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCAGATTAACGTGTATA645100.17685340157957422No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC643380.17638186561504643No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG634220.17387066245511945No Hit
GAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTG608020.16668796346056844No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG593060.16258669716444316No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG581570.15943672726187097No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATCAGATTAACGTGGAGA565160.1549379451816961No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA565060.1549105303000375No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA557170.15274749613717464No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA537090.14724258790012945No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG536360.14704245926402174No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT533380.1462254957905957No Hit
CGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTA521200.14288636320457926No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG518380.14211326354180695No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT509680.1397281688375095No Hit
CGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCC490340.13442613072473789No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT487550.13366125552646319No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA476530.13064013556768642No Hit
CTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATT470410.1289623448101806No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG455690.12492687423003591No Hit
GGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTA444040.12173304051681No Hit
GCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGC435510.11939455111133214No Hit
CCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATAT432350.11852824085092065No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG426150.11682851818808797No Hit
TATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGC424470.11636794817622363No Hit
AGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGG409290.11220636914044942No Hit
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG408640.11202817240966859No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC397710.10903172584438453No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG396370.10866436643015939No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA395460.10841489100706621No Hit
ATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTA394470.10814348367864615No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG394440.10813525921414859No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG393420.10785562742123093No Hit
TGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTG388060.10638618976433045No Hit
GGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCA385030.10555551885007511No Hit
TATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACA384850.10550617206308965No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA381020.10445618209556559No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT369520.10130347070482755No Hit
GGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCC369290.1012404164770128No Hit
CATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCC368640.10106221974623196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)