FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66652280
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGACGTTGATCTCG13898412.0852114886392483No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT4722820.708575910681525No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT4427910.6643298623843026No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTGACGTTGATCGCG4395300.6594373065707579No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT4258910.6389743906735074No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3602820.5405396484561368No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG3358760.5039227465287008No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA2849700.4275472646997222No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA2842140.4264130199297008No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA2647630.3972302222819684No Hit
GGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAG2524970.3788272509207487No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC2457250.3686670583511922No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT2454810.36830097935134404No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA2449880.3675613197327983No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA2352460.3529451655667293No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG2260910.3392097014535737No Hit
GCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGA2150400.32262962347274543No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT2084240.31270348141128856No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG2046150.30698874817185545No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT1991930.2988539926916228No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT1957010.2936148620872384No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA1826980.2741061521076248No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC1796220.26949115619150615No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC1655830.2484281107863077No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC1625530.243882129763603No Hit
GCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATG1611020.24170515997352227No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACC1593770.23911710147049733No Hit
CGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAA1512590.2269374731066964No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG1512510.22692547051653744No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT1508480.22632084003728006No Hit
GTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAAT1492850.22397583398497395No Hit
GCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGG1458970.21889273705265597No Hit
CGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCA1411210.21172719072775903No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1350000.2025437089323876No Hit
CACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGG1304890.1957757484065061No Hit
CCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGA1274380.19119826058463416No Hit
GTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGT1271830.19081567802331745No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC1248680.18734242849606944No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG1241640.18628620056208128No Hit
TATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATC1231430.1847543699930445No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG1204600.18072900131848454No Hit
CCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAAT1197900.17972378439267195No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC1185100.17780336996723892No Hit
GGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGC1180540.17711922232817842No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC1177870.17671863588162326No Hit
CCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATA1176210.17646958213582492No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG1143580.1715740256747406No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT1136060.17044578219979872No Hit
CCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC1110150.16655844331206676No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT1105720.16589379988201453No Hit
CGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGG1083810.16260659050223036No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG1075450.16135231983061946No Hit
CGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGG1070150.1605571482325886No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT1069970.16053014240473093No Hit
CGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACG1055200.15831416419663363No Hit
CCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGAC1039180.15591064551730263No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1018440.15279897401859321No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA984580.14771887773381495No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC984080.14764386154532147No Hit
GGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATAT944810.1417520901010438No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC937440.14064635148264995No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA933740.14009123168779822No Hit
CCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAC930050.13953761221671634No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC910510.13660597957039128No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG891400.1337388608461706No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC890670.13362933721097012No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT879580.1319654781501848No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT833080.12498897262029146No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG832080.12483894024330451No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC828460.12429582303861172No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC824230.12366118608395692No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC818600.12281650380152037No Hit
GGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAA813140.12199732702317159No Hit
TCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAA812380.12188330241666152No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA811560.12176027586753221No Hit
TCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCT809650.12147371402748713No Hit
GCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATT805930.12091559358509565No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC800600.1201159210157552No Hit
GCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACG797350.1196283157905476No Hit
GCGGAGGTGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATAT797020.11957880510614191No Hit
CATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACC794170.11915121283172908No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG778110.11674169285731861No Hit
GGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTT764790.11474326159585238No Hit
CCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTAC762910.11446120072711691No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC756760.11353850160864713No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG754650.11322193329320468No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC753980.1131214116006234No Hit
GCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC752010.11282584781795912No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC745570.11185963931016313No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA742230.1113585311710267No Hit
GTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGAC741490.11124750721205635No Hit
GCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCG735920.11041182687223904No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA728100.10923857368420106No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT726690.10902702803264944No Hit
TGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTT723030.1084779095328772No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA719510.10794979556588312No Hit
GGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATC712350.10687556374665652No Hit
CGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGAT707210.10610439732894358No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG704420.10568580699715No Hit
GTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTC703660.10557178239063988No Hit
GCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGG701640.10526871698912627No Hit
TGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTT699560.10495664964499339No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG699310.10491914155074664No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT698440.10478861338276801No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC697420.10463558035824132No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT692490.10389592073969563No Hit
TGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGC688250.10325978346127093No Hit
CCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGAC686520.10300022744908352No Hit
GGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC681510.10224856524037888No Hit
GATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCG673480.10104380525317364No Hit
CTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCC673230.10100629715892691No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA673000.1009717897122199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)