FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44799237
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATAACCGATCTCG5725381.2780083732229635No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT2404330.5366899440720385No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT1789030.3993438548964573No Hit
GGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATAT1490190.33263736165863717No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1396560.31173745213562454No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTAATAACCGATCGCG1311300.2927058780041276No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG1273280.2842191263212809No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT1089280.24314699824017094No Hit
GCGGAGGTGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATAT980600.21888765650182837No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT933910.20846560400124672No Hit
GTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGA906410.20232710659781997No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA900620.2010346738717894No Hit
GTGGAAGTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG881870.19684933473308933No Hit
GGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATATC837570.1869607734613873No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG813010.1814785372349087No Hit
GTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATATCGGGGGCACCG791860.17675747468645503No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG763440.17041361664262275No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA747020.16674837564755846No Hit
CTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAA745810.16647828176180768No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA731850.16336215726174086No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA728000.1625027676252611No Hit
GTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGA699780.15620355319890827No Hit
GGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTT689020.15380172657851293No Hit
GTGGGGATAGCGATGATTATGGTAGCGGAGGTGAAATATGCTCGTGTGTC675540.15079274676039683No Hit
GTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCTA674810.15062979755659678No Hit
GGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAG671080.1497971940906047No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC659750.1472681331603929No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA650790.14526809909731275No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA643540.14364976796368206No Hit
GAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGG627050.13996890170249998No Hit
GTGGAGTGTGGCGAGTCAGCTAAATACTTTGACGCCGGTGGGGATAGCGA620350.13847334051693783No Hit
GGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATATCGGG616900.13770323811541701No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT599880.13390406626791435No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA599380.13379245722421568No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA592660.13229243167690558No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCTACAACGT591520.1320379630572726No Hit
GTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTC588060.1312656284748778No Hit
GTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCC576610.12870978137417832No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA574980.12834593589172066No Hit
GCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAAT565140.12614946991173087No Hit
GGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTT560330.12507579091134965No Hit
GGACATAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATG554870.12385702015416022No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT539780.1204886592153344No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT536480.11975203952692319No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG526480.11751985865294982No Hit
GCCGGGGAGAGCGAGCGGGGCCGTGCCCGGCGGCGCGGAGCGGCGCGGCG525710.11734798072565387No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT519830.11603545837175754No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT514920.11493945756263663No Hit
GCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGA514460.11483677724243384No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT514410.11482561633806398No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA497320.11101081922444349No Hit
GGTGGGGATAGCGATGATTATGGTAGCGGAGGTGAAATATGCTCGTGTGT495790.11066929555072556No Hit
GCGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGT495560.11061795539062418No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG494590.11040143384584876No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT484250.1080933588221603No Hit
GTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGA476130.10628082795249391No Hit
GGGGATAGCGATGATTATGGTAGCGGAGGTGAAATATGCTCGTGTGTCTA473390.10566921039302521No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA466490.1041290055899836No Hit
GGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGC463590.10348167313653132No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG459610.10259326514868991No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC448200.10004634677148631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)