FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61191641
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT5101000.8336105907014326No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGG3918310.6403341920508391No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT3507490.5731975712172844No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG3259060.5325988887926703No Hit
AGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGC3127840.5111547833796449No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG2518350.41155130976141No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA2424350.3961897344769688No Hit
GGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCC2280570.3726930611323203No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT2212690.3616000427247898No Hit
TGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTG2161380.3532149105136762No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG2020710.3302264765215236No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT1989030.32504929880863953No Hit
GCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAG1930840.3155398300235158No Hit
GGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGC1811880.2960992662380144No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1808800.2955959295159285No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATG1736580.28379366390909505No Hit
GGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTA1697510.27740880490523206No Hit
GGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAA1671000.2730765138329923No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC1668430.27265652182787514No Hit
GTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCT1525470.24929385371443136No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1482860.24233048432219687No Hit
GGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGAT1422150.23240919458263914No Hit
GAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATC1418530.2318176105131745No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC1405490.22968660049499245No Hit
GCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCT1400240.22882864017325505No Hit
TGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGG1369940.2238769834592277No Hit
AGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCT1339840.21895801094793324No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC1322260.21608506952771536No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT1299640.21238848619862966No Hit
GCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGG1264050.20657233232231834No Hit
GACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCC1205900.19706940037774115No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCC1132300.18504161377205097No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA1129640.18460691387570405No Hit
GAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATG1127180.18420489818208993No Hit
GCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTA1099740.1797206255671424No Hit
GCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCG1092030.17846064955179092No Hit
GGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCG1083850.1771238656600172No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA1083080.1769980314794957No Hit
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCC1061000.17338969549778865No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA1056200.17260527463220016No Hit
CGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGAT1049580.1715234275217427No Hit
GTGCGGTAACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGA1046760.17106258026320947No Hit
GGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTG1043100.17046445935319826No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT1024370.16740358376726652No Hit
GGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGG1018310.16641325242446103No Hit
GAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCAC1016600.16613380249109513No Hit
GAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTG1011720.16533630794441354No Hit
GGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC995810.162736279617015No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC988960.16161684567341478No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA982880.160623245910336No Hit
CAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCC980020.1601558618112562No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAG970660.15862624112335866No Hit
GCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCG970040.1585249200948868No Hit
GGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTG947110.15477767625156513No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAG945680.15454398420202523No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG935660.15290650564510927No Hit
CGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTA908190.14841733039975183No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG896630.1465281834817929No Hit
CTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGG894690.14621114671528418No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCT871430.14240997393745333No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG866850.14166150569487096No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG848650.13868724324618129No Hit
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGG841840.13757434614312763No Hit
GACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCC839180.13713964624678066No Hit
CTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAAG832400.13603165177413692No Hit
GGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTC826100.13500209938805205No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA823040.13450203108623937No Hit
AGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGC814950.13317995508569544No Hit
ACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTT810710.13248704998775895No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG808990.13220596584425642No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT804780.13151796337672983No Hit
GAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGGG802850.1312025608203578No Hit
GATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAG800010.13073844514155128No Hit
GCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGC794310.12980694536366494No Hit
GGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTG794050.12976445590011224No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG786810.12858128776118294No Hit
GGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGC784430.12819234574866198No Hit
GGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCC779490.12738504594116049No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT773320.12637673828685195No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC768860.1256478805659093No Hit
GAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGGAG765850.12515598331477987No Hit
GTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATG762630.12462976765078093No Hit
CCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT746520.12199705512064957No Hit
GGCGCGGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCA743070.12143325262350783No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG739240.1208073501411737No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC731630.1195637162271886No Hit
GGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGGA728520.11905547687469274No Hit
AGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGC726080.11865672960135192No Hit
GGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGA725210.118514553319464No Hit
GGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGA720230.11770071667141595No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT717960.11732975097039806No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG713460.11659435640890886No Hit
GGTAACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTC697360.11396327808891413No Hit
AATTAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCT694170.11344196505532513No Hit
TAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAAGCA685630.11204634959863227No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAG685570.1120365443378124No Hit
GGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAG685540.1120316417074025No Hit
AAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAGATT684800.11191071015729093No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG684460.11185514701264508No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG678060.11080925252519376No Hit
GCCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATG666480.10891683718696153No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA657780.10749507436808241No Hit
GCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGG646080.10558304850821047No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG644990.10540491960331641No Hit
GAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCT635340.10382790682145623No Hit
GGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCG629710.10290784651452638No Hit
GTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGG629600.10288987020302331No Hit
TACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGG619940.10131122321102648No Hit
GACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCG617970.10098928381410788No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG616660.10077520228620768No Hit
GGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAG616160.10069349177937556No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG614420.10040913921559973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)