FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71290333
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG8242031.1561216862319887No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT7311841.025642565030521No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATCGGACCGGTGGTGT6243960.8758494647514131No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT4995550.7007331555037063No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT3800740.5331353971933334No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATCGGACCGGTGGTGG3719610.521755172612253No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG3656730.5129349024081569No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATCGGACCGGTGGGGG3550200.4979917824202056No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATCGGACCGGTGTAGA3515950.49318748447983823No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG3171800.44491305714619117No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT2812550.39452053057460124No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG2623000.36793207292214497No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG2606560.3656260099107687No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA2514610.35272804799495044No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA2423820.33999280098747753No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC2312170.32433149105924364No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATCGGACCGGTGGGGT2087370.2927984639937087No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1952130.27382815002421157No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT1806470.2533962073090611No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1794960.25178168265815226No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG1746110.2449294212161977No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG1736890.2436361182377981No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA1703530.238956661908144No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATCGGACCGGTGTTGT1653170.23189259054239514No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGG1630330.2286887901056655No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1568040.21995128007046902No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG1533270.21507404096429175No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCC1508830.2116458061712238No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT1489570.2089441776068012No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1463980.20535463062011505No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1452780.2037835901257468No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC1439770.20195865826577075No Hit
GGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCCCCGGTG1430780.2006976177260948No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC1429310.20049141866120895No Hit
GTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCCCCGGTGG1405630.1971697901874017No Hit
GGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCG1320020.1851611494085741No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA1295430.18171187389459942No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG1261090.17689495152168808No Hit
TGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG1258410.1765190239748214No Hit
GAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTC1254060.17590884306852655No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC1253530.1758344991879895No Hit
GGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGC1239530.17387069857002913No Hit
GAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATC1230490.17260264445671758No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA1220870.1712532328892334No Hit
GGGAAGGGTGCCGGCGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGC1220640.17122097045050974No Hit
GGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGG1214790.17040038233514773No Hit
GAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCCC1206110.1691828259520123No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT1186000.16636196663578495No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA1183390.16599585809200806No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG1165230.16344852814756805No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1160990.16285377710327148No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATCGGACCGGTGTAGT1155860.16213418444826175No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT1108820.1555358143719149No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT1062790.14907911848300667No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG1046570.14680391519562688No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT1030700.1445778069236961No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA988170.13861206118927794No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG977500.1371153645754467No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT974030.13662862256513797No Hit
GGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTG969070.13593287606048915No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG962340.13498884904914107No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC957660.13433237855685146No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA951370.1334500709934964No Hit
ATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT939230.13174717531477934No Hit
TGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTG934820.13112857812012185No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG931320.13063762796563175No Hit
GTCGTCCGGCCCCGCCGCGCCGGCTCCCCGTCTTCGGGGCCGGCCGGATT929240.13034586330239192No Hit
AGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCCCC880290.12347957471316622No Hit
AGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCC861960.12090839861836526No Hit
GGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTA842030.11811278816722598No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC839740.11779156649471675No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC828220.1161756391290808No Hit
GAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATC823190.11547007362134219No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA802490.11256645413621508No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATCGGACCGGTGTTGA797180.11182161261611724No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA774450.10863324204138589No Hit
GTGCCGGCGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTT767270.10762609286731764No Hit
GAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCC762460.10695138708357556No Hit
GGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCG760900.10673256358614569No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA750580.10528496198776346No Hit
GGAAGGGTGCCGGCGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCC744900.10448822002276242No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATCGGACCGGTGGAGT741230.10397342371791136No Hit
GGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCC740040.10380650066538476No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT733630.10290736052530432No Hit
GGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGC725520.10176975888161442No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATCGGACCGGTGGTGA724990.10169541500107736No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC723420.10147518878892037No Hit
GGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGA722300.10131808473948353No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG715330.10034039257468469No Hit
GGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCC713710.10011315278889216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)