FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63423720
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAATACCTATATCTCG40540966.392081700663412No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAATACCTATATCGCG10762001.6968414971559536No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT3412050.5379769587781984No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG2710520.4273669220285407No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT2553240.4025686288978319No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT2103320.33162986970805247No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA1842500.2905064540522063No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA1825790.2878717930767858No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1804700.2845465387397649No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA1754780.2766756664541279No Hit
GGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAG1688350.26620166713652244No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1586490.25014142973638254No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG1572970.24800973515902253No Hit
GTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAAT1540700.24292173338303086No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1512300.23844391341283672No Hit
GCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGA1484900.23412376315990296No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC1358280.21415962356039664No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT1333630.21027306502992885No Hit
GGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATAT1315000.20733567819736842No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC1313570.2071102105016861No Hit
GCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATG1302630.20538530379485784No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC1263540.19922199454715048No Hit
GGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTA1261490.19889877162676678No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT1250850.1972211658351166No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG1232460.1943216197346986No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACC1199230.18908225503013698No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA1145090.1805460165376613No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT1140270.1797860485004664No Hit
GCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGG1077400.1698733533763078No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1074610.1694334548651514No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG1057830.1667877570095226No Hit
GCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGG1052270.16591111338155504No Hit
CGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAA1041850.16426819492770214No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC1026460.16184165797906525No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG1016270.1602350035601822No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT996910.1571825178340217No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT974260.15361129873807464No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA973780.15353561727378967No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA973340.15346624259819514No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG945090.14901207308559006No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT933880.14724459555510147No Hit
GCGGAGGTGAAATATGCTCGTGTGTCTACGTCTATTCCTACTGTAAATAT905400.14275416200752652No Hit
GCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCG905220.14272578145841966No Hit
GAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGG900250.14194216296363568No Hit
GTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCC879260.1386326755983408No Hit
CCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAAT874890.13794365893391305No Hit
GGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGC874500.13788216774418152No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT857960.13527431062069523No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG846360.13344534190047508No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA845670.13333654979556545No Hit
GGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTT843230.13295183568545016No Hit
GGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATC829630.13080752753070932No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT816780.12878147166391377No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT813370.12824381792805595No Hit
GGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATATCGGG807210.12727257246973214No Hit
CGCGCCGCCCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGG798730.1259355332673643No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC797740.12577944024727655No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC788170.12427054105309497No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT784790.12373761740875495No Hit
GTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGA782230.12333398293256845No Hit
CCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGA778820.12279632919671064No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG774210.12206947180014038No Hit
CGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCA769140.12127008633363039No Hit
TATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATC762350.12019950895343257No Hit
GATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCG759800.11979745117441866No Hit
GCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCT753050.11873318058291124No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG751050.11841784114839055No Hit
GCCGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCC739730.11663301994900331No Hit
GTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGT737080.11621519519826337No Hit
GTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT723120.11401412594530878No Hit
GGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATATC719800.11349066248400441No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA716280.11293566507924796No Hit
GTGGAGTGTGGCGAGTCAGCTAAATACTTTGACGCCGGTGGGGATAGCGA714480.1126518595881793No Hit
CCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC708030.11163488991185003No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC705790.11128170974518681No Hit
GTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCTA698390.11011495383746019No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA691070.10896081150711437No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC689500.10871327005101561No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC688050.10848464896098811No Hit
GTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATATCGGGGGCACCG687800.108445231531673No Hit
CCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATA687680.10842631116560175No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG679460.10713026608972163No Hit
GCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACG677580.10683384702127217No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT674120.10628830979955134No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT672020.10595720339330458No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT659810.10403205614555562No Hit
GTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGA646240.1018924780823326No Hit
CACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGG643030.10138635828992686No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)