FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63423720
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGTGTAGA11953881.884764879764227No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGTGTTGT8074501.2731041320187464No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGTGGTGT5687350.896722866460687No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGTGTTGA4431670.6987401558912029No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT4114240.648691057541248No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGTGTTTT3453570.5445234054388485No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGTGTAGT2828500.4459687952709176No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGG2511790.3960332191173902No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT2121430.33448526828763747No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1979660.3121324324716368No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1743460.27489084525474067No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1475360.23261959405723914No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGTGGTGA1411240.2225098117865051No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT1408240.22203680263472403No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGTGGTTT1402380.22111285809157835No Hit
GGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGC1374520.21672017976870483No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT1370800.21613364842049632No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG1323670.20870267464601572No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGGGGTGT1307160.20609954761404725No Hit
GCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGC1302970.20543891149872634No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG1256900.19817506762454173No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT1253610.19765633425475518No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG1244890.19628145432024485No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA1232240.19428693239690137No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA1219260.19224037946686193No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGTGTTTA1194280.18830178992969823No Hit
TGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTG1136480.17918848027204962No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGTGTATA1132420.1785483412199726No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG1125900.17752033466343506No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC1123770.1771844981656705No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT1090780.17198297419325137No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT1084180.170942354059333No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG1055090.16635574198422926No Hit
GGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTA1053610.1661223908026839No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG1029900.16238404180644087No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT1024430.16152158845302672No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGGGTTGT1016990.16034852575660966No Hit
GGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCC1016550.16027915108101512No Hit
GGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCG1008500.15900990985706923No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA948600.1495654937931739No Hit
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG925030.1458492185573473No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA917760.1447029597128645No Hit
GGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTG895880.14125314629920793No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGTGTTGG860640.13569686546295298No Hit
GGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCC847220.133580937857319No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAG837360.13202631444513188No Hit
CGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCC835550.13174093225689062No Hit
GGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGA819410.1291961430203085No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT810280.12775661850172143No Hit
GAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTG809850.12768882052329947No Hit
AGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGC803120.1266277033261373No Hit
GCCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATG802090.12646530351735913No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAACTCTATTGTGTGGTGG795940.12549563475620792No Hit
GTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCT786240.12396623849878247No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG784500.12369189319074946No Hit
GAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATC784080.1236256719095001No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC780450.123053330835845No Hit
CGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTA778700.1227774088306394No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA773780.12200167382171845No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC760850.11996300437754202No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG738670.11646589004870732No Hit
GGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGC724600.11424747712685411No Hit
GGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTG723030.11399993567075535No Hit
TATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGC703170.11086861508596468No Hit
GCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTA692960.10925880727273646No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC692030.10911217443568431No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC684500.10792492146471384No Hit
GTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCC676220.10661941620579808No Hit
GCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCG668830.10545423699524406No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC667640.10526661003170423No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT664330.10474472326757246No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA664190.104722649507156No Hit
GGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC662120.10439627319242706No Hit
TACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGG658510.10382708551311719No Hit
GGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGAT639340.10080455703323614No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)