FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57075041
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGATATGACAATCGTGGGGG13375292.3434569236665115No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT3362920.5892102644306466No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATGACAATCGTGGGGT1805430.3163256597573009No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT1716410.30072864949847344No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1626830.2850335227967685No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGG1574000.27577728765889103No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT1552970.2720926648129784No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT1534110.2687882431832156No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA1493170.26161523037714507No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG1436020.2516020969656421No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC1366010.2393357895266339No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATGACAATCGTGGTGG1350790.23666912477557397No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1249450.21891355277344435No Hit
GGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGC1197260.20976945071314096No Hit
GAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTG1164120.2039630597900052No Hit
AAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTG1161870.20356884193915864No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATGACAATCGTGGTGT1135740.19899065863132714No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG1066720.1868978070466914No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT1049850.18394204920501064No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT1048030.1836231707656592No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATGACAATCGTGTGGG1042250.1826104689088178No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT1033780.1811264577102976No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT1017580.17828808918420225No Hit
GGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTA1000810.17534985213589246No Hit
CGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTA990080.17346987100718858No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG979330.17158638571981052No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA954610.16725524559850952No Hit
TATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGC944070.16540855397721047No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA908240.15913085371239594No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG902760.1581707142356674No Hit
GGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTG893760.15659384283228112No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT848360.14863940264186581No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT812390.14233717326633194No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATGACAATCGTGTTGT799510.14008049508015247No Hit
GGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCC779380.13655355937457847No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC778110.1363310452987673No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG773310.13549004721696126No Hit
GAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATC760100.1331755504126576No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG755450.13236083352090802No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA750100.13142347107556174No Hit
CGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCC742130.1300270638438963No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA741770.12996398898776088No Hit
GGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCG731250.12812080152513602No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT728060.12756188821660241No Hit
GCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGC724450.12692938757591082No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT723180.12670687350009963No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG719630.1260848853354306No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA716990.1256223363904373No Hit
GGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCC713550.1250196210984763No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA703280.12322023561927883No Hit
GGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGA693290.12146990836152005No Hit
GGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGA693060.12142961053676685No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG689770.12085317643486232No Hit
GGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGAT686220.12023118827019327No Hit
AGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTATTCGAGCCGAGCTGG681630.11942698385446628No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATGACAATCGTGTGGT678320.11884704559388752No Hit
CCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATAT676750.11857196913796347No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC675280.11831441347541038No Hit
ACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAA664530.11643092818803231No Hit
GTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCC651920.11422155614395442No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATATGACAATCGTGTAGA651550.11415672920848186No Hit
TATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACA640200.11216811916087806No Hit
GAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCT637370.11167228070847991No Hit
CATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCC635310.11131135236503815No Hit
TGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTG630850.11052992498069339No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC626720.1098063162144728No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG620350.10869024167674272No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA605490.10608665178181825No Hit
GGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGC604280.10587465018202967No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA597240.10464118632871415No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG591070.10356015337772599No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA575400.10081464505649676No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC574430.10064469336079845No Hit
GGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTG573990.10056760186996626No Hit
GTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACC571860.10019440897116483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)