FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44840624
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG4746401.0585044490014235No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT3816910.8512169678994654No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT3257100.7263725857160239No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA2930420.6535190054447056No Hit
GGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGC2465430.5498206269386439No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC2444850.5452310387116824No Hit
TGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTG1768360.39436560918509966No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG1702150.37959998058903016No Hit
CGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTAC1642880.3663820557002061No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG1547470.3451044749065044No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC1525500.3402048999139709No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA1365120.30443822548053745No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA1339940.2988227817703875No Hit
GCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTA1317190.293749257369835No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1309940.2921324199235051No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG1270210.28327215071761713No Hit
TGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGG1213230.2705649234497718No Hit
CGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCT1201340.26791331003779073No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG1157670.25817437330934556No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA1023350.22821939320023735No Hit
GCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGG1007010.22457537611430206No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG1004600.22403791704593587No Hit
CGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCG1001180.22327521579539128No Hit
CGCCTCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGTGCGGACCAGGGG995880.22209325186910867No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG977110.2179073154735759No Hit
AGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGC971590.21667628889374957No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGG967900.21585337438658303No Hit
CCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTG934500.20840477153038725No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA930150.2074346690625893No Hit
CTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGG907950.20248380129589635No Hit
GTGCGGTAACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGA886920.19779385764123175No Hit
TACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGG875840.19532288399911651No Hit
CCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGC869800.19397589114727753No Hit
CCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT868260.1936324525724709No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC849650.18948219810678818No Hit
CGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGC840490.1874394076228734No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA825330.18405854476958214No Hit
CGCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGG824620.1839002062058726No Hit
GACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCC810700.18079587830891916No Hit
CTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAAG788830.17591860452254188No Hit
CGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTA785030.17507115868860343No Hit
TACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGG775850.17302390796345743No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT769350.17157432956329954No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT761050.16972332945232876No Hit
CTCGCCTCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGTGCGGACCAGG728530.16247097721030823No Hit
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCC715850.1596431842696926No Hit
CCGGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGG709850.1583051119003161No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG698240.1557159418655726No Hit
CCTCGCCTCGGCCGGCGCCTAGCAGCCGACTTAGAACTGGTGCGGACCAG674590.15044170660961365No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG672190.14990647766186305No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA668980.14919060894424663No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG667560.14887393181682754No Hit
GGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTA665190.1483453932309238No Hit
TGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCA638570.1424088121521235No Hit
CCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACC632000.14094362290765625No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAG621500.1386019962612474No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT618670.1379708721270248No Hit
TGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAG612530.13660157806902956No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC592930.1322305416623997No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT585060.13047543673790088No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA580160.12938267763624342No Hit
AGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCG578670.12905038966451493No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA576100.12847724866629867No Hit
CCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGC571370.12742240161510687No Hit
GCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGG570670.12726629317201296No Hit
CCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCG568070.1266864618119498No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG566260.12628280998052124No Hit
ACCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCA561140.12514098822531997No Hit
GCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGC559330.1247373363938914No Hit
CTAATTAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTAC558570.1245678472271037No Hit
GACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACA558120.12446749179940046No Hit
CTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAG557840.12440504842216289No Hit
GCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGC556460.12409729177720631No Hit
ATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGG553710.12348400860790876No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC548370.12229312419916369No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAG545690.12169545187417552No Hit
GCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAG537130.1197864686271984No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGG534730.11925123967944781No Hit
CCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA532600.11877622398831916No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCT532190.11868478904307844No Hit
CGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTT530670.11834581070950306No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCG524180.11689846242996084No Hit
TCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCG523190.11667768048901372No Hit
AATTAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCT517280.11535967920517788No Hit
CGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCA516210.11512105629930573No Hit
ACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGG513940.11461481891955831No Hit
CGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGT512500.11429368155090795No Hit
GGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAG510500.11384765742778245No Hit
GCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTC508680.11344177547573825No Hit
CGGGTGGAGCCGCCGCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCA500630.11164652838015812No Hit
GCCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATG498260.11111798979425443No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG492580.10985128128457801No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA490420.10936957523160248No Hit
CGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCG490190.10931828245744304No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC490160.10931159209559617No Hit
TGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAAT487780.10878082338907683No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT486090.10840393300503579No Hit
CAGGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGG485260.10821883299393872No Hit
ACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTT485110.10818538118470429No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT484830.10812293780746672No Hit
GGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCC478550.10672242206085268No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA476560.1062786280583428No Hit
CCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCT475290.10599540274015812No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC473400.10557390994380453No Hit
GCCTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGAT469120.10461941832031597No Hit
AAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTG464690.103631474887593No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG463680.10340623270541462No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC457450.10201686756187872No Hit
ACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACAC455770.1016422072984533No Hit
GAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCT455560.10159537476552513No Hit
CGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCT454480.10135452173903735No Hit
GGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTG454070.10126308679379663No Hit
TCTAATTAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTA453180.10106460605900577No Hit
CAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCC449560.10025730239614863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)