FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63706181
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT4286120.6727949992795832No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG4173150.6550620260850356No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT3341690.524547217796653No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG2511670.3942584472297908No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA2461770.38642561229655253No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG2452180.3849202638594833No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG2451120.38475387498114194No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG2340970.3674635589912382No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT2307630.36223015785548346No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1968010.3089197891174798No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG1766170.27723683515105074No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC1759690.2762196654042094No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGG1721460.27021867783912523No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG1664930.2613451275630539No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT1613230.25322974547791527No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC1528200.23988253196342127No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT1498680.23524875867225506No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG1479850.2322930015220972No Hit
TGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTG1332500.20916337772625232No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1319210.20707723792138788No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATG1310440.20570060540907326No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1308060.205327015286005No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA1284650.2016523326049006No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG1242930.19510351750640964No Hit
GGGCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGC1217820.19116198473739934No Hit
CGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGAT1162090.18241401097328372No Hit
AGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGC1149970.18051152681715452No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCC1134270.17804708777002345No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG1119660.17575374672043204No Hit
GGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTG1114390.1749265114479237No Hit
GCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCT1107610.17386225050909895No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT1105990.1736079580723886No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1094590.17181849277701955No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA1082090.16985635977771135No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1039180.16312074961768624No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA995300.1562328779369148No Hit
GGGAGGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTA993640.15597230667460665No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA989620.15534128470202915No Hit
GGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGAT987990.15508542255891936No Hit
GCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTA984840.1545909650430937No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG982830.15427545405680496No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG955990.1500623620806904No Hit
GCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGC953830.14972330549840998No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC944690.1482885938493158No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG922580.1448179730001395No Hit
GAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCAC920460.14448519524345682No Hit
GCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGG910240.14288095530322248No Hit
CCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTT909470.14276008791046507No Hit
CAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCC908740.14264549934330548No Hit
GCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCG897520.14088428876312645No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA889730.1396614874779576No Hit
AGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCT875140.1373712858411651No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT871110.13673869416218815No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA864120.135641469388975No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCC857260.13456465079895466No Hit
TGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG852580.13383002820401366No Hit
GTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCT848280.13315505445225168No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG831870.13057916625075988No Hit
AGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT830910.130428474436413No Hit
GGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGG824490.12942072292796833No Hit
GTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATG820280.12875987653380133No Hit
CCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTG805490.1264382807690199No Hit
GGTGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCC804670.12630956484426528No Hit
TGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC791610.1242595282865881No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG790500.12408529087624952No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC780210.12247006299121901No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG753050.1182067404103222No Hit
GCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGC747440.11732613512023268No Hit
GGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCC734740.11533260799293557No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC729750.11454932449961173No Hit
GAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTC716970.11254323972111906No Hit
GCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGG715690.11234231730198989No Hit
CGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGC712820.11189181156534875No Hit
GGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGC710960.11159984617505167No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAG710370.11150723349748434No Hit
GGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAG706690.11092958154248801No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT706360.11087778123130627No Hit
GGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAA705530.11074749560015221No Hit
TGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGG700710.10999089711561896No Hit
GAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTT698090.10957963403896397No Hit
GGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGA693260.10882146584803129No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT671280.10537125118204778No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA667890.10483912071263542No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG661120.1037764294802101No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA660220.1036351559042599No Hit
GCGCGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGG646950.10155215551219433No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)