FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83502413
Sequences flagged as poor quality0
Sequence length50
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGGAGGACGGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGG5042410.6038639865413231No Hit
GGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGC3909100.4681421601552999No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT3487000.4175927227396411No Hit
GGCGAGGAGGACGGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAG3380640.40485536627546326No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT3247280.38888457031774637No Hit
GCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCC3176200.3803722414584594No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG3125730.37432810474590716No Hit
GGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGG3030540.3629284341759082No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT2850240.3413362437801648No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG2699520.3232864659851207No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT2585870.30967608085768733No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2397950.28717134198265626No Hit
GCCGGGGAGAGCGAGCGGGGCCGTGCCCGGCGGCGCGGAGCGGCGCGGCG2060900.24680723897164505No Hit
GGGAGGGCGAGGAGGACGGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGG2033180.24348757442494506No Hit
GGCAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGC2031690.24330913646770902No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1999360.2394373920667418No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1974520.236462627732686No Hit
GCGGGAACGACACACCACCGTTCGGCCTCGGGCACCTGAGGGACAACCCG1897500.22723894218482044No Hit
GGGAGAGCGAGCGGGGCCGTGCCCGGCGGCGCGGAGCGGCGCGGCGGAGG1839420.22028345456316334No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1795540.21502851660107117No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1705050.20419170401698453No Hit
GGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCG1679690.20115466603342347No Hit
GGGGAGAGCGAGCGGGGCCGTGCCCGGCGGCGCGGAGCGGCGCGGCGGAG1608680.19265072016541607No Hit
GGGGTCTTTAAACCTCCGCGCCGGAACGCGCTAGGTACCTGGACGGCGGG1594030.1908962798476255No Hit
GCCGGGAGAAGACGAGAGACCACGGGCGAGGCCGGGGCGACGGGGAAGGC1591420.19058371402991672No Hit
GTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCACCG1488940.17831101479666223No Hit
GGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCG1476680.17684279375255899No Hit
GGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGC1437770.17218304817131452No Hit
CGCTAGAGAAGGCTTTTCTCACCGAGGGTGGGTCACACTCCCCCCACCCG1433840.1717124030894772No Hit
GGGGGACGCGCCGACCCGGTTCGGAAGAGCGGGCCGGGAGAAGACGAGAG1401630.16785502953070353No Hit
GGGGCGGGAACGACACACCACCGTTCGGCCTCGGGCACCTGAGGGACAAC1367370.1637521540844574No Hit
GCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCC1332680.15959778312035128No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1293850.15494761810056915No Hit
CGGGAGAAGACGAGAGACCACGGGCGAGGCCGGGGCGACGGGGAAGGCGC1288660.1543260791757No Hit
CGAGAGACCACGGGCGAGGCCGGGGCGACGGGGAAGGCGCGAGAAAGGCG1274870.15267462989362954No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1271780.15230458070714675No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT1248390.14950346404959577No Hit
GCCGGGCGGGCGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACG1244420.14902802868702728No Hit
CCACACACGACCGGTCGGAGGCAGAACGGCAGCCCCTCGGCGGCCGGCCG1242890.14884480044906007No Hit
CCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG1228280.14709515041200066No Hit
GGGGGCGGGAACGACACACCACCGTTCGGCCTCGGGCACCTGAGGGACAA1215070.14551316020053215No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC1202310.14398506064728933No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT1160860.13902113223961562No Hit
GGGCGAGGAGGACGGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGA1160460.13897322943230395No Hit
GGCGGGAACGACACACCACCGTTCGGCCTCGGGCACCTGAGGGACAACCC1155260.138350492937252No Hit
CGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGA1118640.13396499092786696No Hit
GGGAAGAAGAGGATCGGCGGGCGGGGAAGAGGGCACAGACGGGCGAGGGC1093620.13096867033052087No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1087700.1302597087823079No Hit
GGGGGAGAGGCGACGGGAGAGAGAGCCGCGGCCGACGGCACCCCCGCGCC1078230.12912560981920368No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1074600.1286908918428501No Hit
GGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAG1073270.12853161500853874No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG1061640.1271388408859514No Hit
GGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGAAGAGGGCACAGACGGG1046640.1253424856117631No Hit
CCGACACGCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACC1044180.1250478833467962No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1037540.12425269674542219No Hit
CCCGACACGCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGAC1028480.12316769815981245No Hit
GCCACGAACCCGACACGCCACCACCACCGTCGCTCGTGATTCTCGTCCAT1013980.12143122139476378No Hit
GGAGGACGGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCG1009070.1208432144350128No Hit
GGGGACGCGCCGACCCGGTTCGGAAGAGCGGGCCGGGAGAAGACGAGAGA997490.11945642816333943No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT995950.11927200235518941No Hit
GGAGGGACCACCGGGCCGCGCTCGGGCGCACGCGCGCGCCGAACGGGGCG987850.11830197050712773No Hit
GCGGGAAGGCCGGGGAGAGCGAGCGGGGCCGTGCCCGGCGGCGCGGAGCG980230.11738942202784007No Hit
GGGAGGGACCACCGGGCCGCGCTCGGGCGCACGCGCGCGCCGAACGGGGC976870.11698703844642191No Hit
CACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGC972880.1165092079434878No Hit
GGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGCGGCG969630.11611999763408035No Hit
GCAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGCC948240.11355839501308782No Hit
GGGTCTTTAAACCTCCGCGCCGGAACGCGCTAGGTACCTGGACGGCGGGG918230.10996448689452844No Hit
GGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGGGAGC906840.1086004544563281No Hit
CCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCG892940.10693583190224695No Hit
CGGGCGCGCGCGCGTACGCGCGGGGAGGGCGAGGAGGACGGGCGGGGCCT890720.10666997132166708No Hit
GGACGGGCGGGGCCTCGGAGGAGGGGCGGCGGGGAGGAGGAGGGGCGCGG881620.10558018245532617No Hit
GTCTCACTCAAACCGCCTCGAACCCCACACCGACGAGCTCCCTCAGGACC863360.1033934193015476No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT862740.10331916995021449No Hit
GGAGAGGCGACGGGAGAGAGAGCCGCGGCCGACGGCACCCCCGCGCCGCC857540.10269643345516255No Hit
GGGACGCGCCGACCCGGTTCGGAAGAGCGGGCCGGGAGAAGACGAGAGAC857400.10267966747260346No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT840660.1006749349866093No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA840060.10060308077564177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)