FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007266434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007266434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83502413
Sequences flagged as poor quality0
Sequence length50
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCCCCGGTG3654940.43770471638945335No Hit
GTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCCCCGGTGG3255580.38987855356946394No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG3011500.3606482605478718No Hit
GGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCG2921410.34985935077109687No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG2820130.33773035995977746No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT2664120.3190470675380363No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG2574940.30836713664789545No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA2476190.29654113109282243No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT2420450.28986587489393867No Hit
GAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCCC2363150.2830037977465394No Hit
GTCGTCCGGCCCCGCCGCGCCGGCTCCCCGTCTTCGGGGCCGGCCGGATT2285630.2737202336895342No Hit
CCCGGCTGCGGTCGGCCGCGCTCGAGGGGTCCCCGTGGCGTCCCCTTCCC2225380.2665048733382112No Hit
GCGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGC2177530.2607745000135505No Hit
CGTCGTCCGGCCCCGCCGCGCCGGCTCCCCGTCTTCGGGGCCGGCCGGAT2093510.2507125153377304No Hit
GGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCC2052340.2457821188951749No Hit
GCCCGGCGTCCGGCCGACCGCCGCTCCCGCGCCCCTCCTCCTCCCCGCCG2027110.24276064932399016No Hit
AGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCC1938560.23215616535536523No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1937260.23200048123160227No Hit
GGCGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCG1867630.22366180004882016No Hit
GTGGGATCCCGCGGCCGTGTTTTCCTGGTGGCCCGGCCGTGCCTGAGGTT1831850.21937689393478962No Hit
GGCGGCTCCGCCGCCGCGTCCTCGGACCCGTCCCCCCGACCTCCGCGGGG1806010.21628237258245459No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG1727190.20684312440168642No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA1690960.20250432762943033No Hit
GTCGGCTCTCCGCCCGCTCCCGTGCCGAGTCGTGACCGGTGCCGACGACC1673020.20035588672150112No Hit
AGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCCCC1658750.1986469540706566No Hit
CGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCC1645310.1970374197449839No Hit
GTGCCGGCGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTT1584250.1897250562088547No Hit
GGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCCCCGGT1542720.1847515472397187No Hit
GGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGC1522940.1823827534181557No Hit
GAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCC1522310.18230730649663981No Hit
GTCCCGGCTGCGGTCGGCCGCGCTCGAGGGGTCCCCGTGGCGTCCCCTTC1497150.17929421991673464No Hit
CGGCCCGGCGTCCGGCCGACCGCCGCTCCCGCGCCCCTCCTCCTCCCCGC1490450.17849184789426384No Hit
GATCCCGCGGCCGTGTTTTCCTGGTGGCCCGGCCGTGCCTGAGGTTTCTC1487180.1781002424444908No Hit
GGCCCGGCGTCCGGCCGACCGCCGCTCCCGCGCCCCTCCTCCTCCCCGCC1478280.17703440498180573No Hit
GCCCGTCGTCCGGCCCCGCCGCGCCGGCTCCCCGTCTTCGGGGCCGGCCG1412330.16913642962629116No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG1392410.16675086982216908No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT1380010.16526588279550675No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1362500.16316893740543761No Hit
GGCGGGGCGGCTCCGCCGCCGCGTCCTCGGACCCGTCCCCCCGACCTCCG1355700.16235458968113892No Hit
GGGAAGGGTGCCGGCGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGC1337260.1601462702640701No Hit
GGCCCGTCGTCCGGCCCCGCCGCGCCGGCTCCCCGTCTTCGGGGCCGGCC1321220.1582253676908714No Hit
GCCGGCGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCG1232840.14764124241535392No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT1212020.14514790129478056No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG1154120.13821396993641372No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1143480.1369397552619228No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG1141680.1367241926290202No Hit
GCGGCCCGGCGTCCGGCCGACCGCCGCTCCCGCGCCCCTCCTCCTCCCCG1114010.13341051593323416No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT1104310.13224887285592574No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1080430.12938907525941795No Hit
CGGCGGGGAGAGAGGGTCGGGGGAGCGCGTCCCGGTCGCCGCGGTTCGCC1034730.12391617952405758No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG1031760.12356050117976829No Hit
CGAGTCGGCTCTCCGCCCGCTCCCGTGCCGAGTCGTGACCGGTGCCGACG1027020.12299285291312481No Hit
CCCGCGGCCGTGTTTTCCTGGTGGCCCGGCCGTGCCTGAGGTTTCTCCCC1020630.12222760556632058No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA1014440.12148630962317221No Hit
GCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTCGCTCGCTCGT1010530.12101805968170046No Hit
TGTGTCCCGGCTGCGGTCGGCCGCGCTCGAGGGGTCCCCGTGGCGTCCCC1003730.12020371195740176No Hit
CCGGCTGCGGTCGGCCGCGCTCGAGGGGTCCCCGTGGCGTCCCCTTCCCC1003490.12017497027301476No Hit
CCCCGCGCGTGTGTCCCGGCTGCGGTCGGCCGCGCTCGAGGGGTCCCCGT994690.1191211085121576No Hit
GTTTTCCTGGTGGCCCGGCCGTGCCTGAGGTTTCTCCCCGAGCCGCCGCC976500.11694272834965858No Hit
GTCCGGCCCCGCCGCGCCGGCTCCCCGTCTTCGGGGCCGGCCGGATTCCC971590.11635472138990761No Hit
GCCGGCGGGGTCCTCTGACGCGGCAGACAGCCCTCGCTGTCGCCTCCAGT969520.11610682436206962No Hit
GGGGAGCGCGTCCCGGTCGCCGCGGTTCGCCGCCCGCCCCCGGTGGCGGC961610.115159546347481No Hit
GGCCGGCGGGGTCCTCTGACGCGGCAGACAGCCCTCGCTGTCGCCTCCAG940050.11257758503338101No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC913200.10936210909258395No Hit
CCCTCCGCCGCGAGTCGGCTCTCCGCCCGCTCCCGTGCCGAGTCGTGACC909720.10894535466897226No Hit
CGACTTGCGGGCGGCCCCCCTCCGCGGCGGTGGGGGTGCCGTCCCGCCGG876580.1049766070831989No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCC867780.10392274532234176No Hit
CCGTCGTCCGGCCCCGCCGCGCCGGCTCCCCGTCTTCGGGGCCGGCCGGA865920.1036999972683424No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC863430.10340180229282715No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGG848750.1016437692644882No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG847060.10144137990359633No Hit
GGCCGGGCCCCCGCCCTGAACGCGAACGCTCGAGGTGGCCGCGCGCAGGT841970.10083181668055509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)