FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12073908
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA8238056.823018694527075RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC8203266.794204494518262RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA3990943.305425219407006No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG3773293.125160469998612RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA3431442.842029275028433RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA3351412.775745847988903RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC3247102.68935294189752RNA PCR Primer, Index 1 (100% over 50bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC2897422.399736688402794RNA PCR Primer, Index 1 (100% over 27bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC1874861.5528195179224489RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA1812481.5011543901113045No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1261681.0449640663155624RNA PCR Primer, Index 1 (100% over 26bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1117990.9259553741837357RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1073040.8887263345057789RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC1069360.8856784398224669RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC1010850.8372185708223054RNA PCR Primer, Index 1 (100% over 26bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA945310.7829362290983167RNA PCR Primer, Index 1 (100% over 28bp)
GCATCCTTAGCTCAGCTGGATAGAGTACTCGGTGGAATTCTCGGGTGCCA908520.7524655645877043No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA882660.7310474785794293RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA785370.6504687628893644RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC771540.6390143108594168RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC769310.6371673529399098RNA PCR Primer, Index 1 (100% over 36bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT720390.5966502312258798RNA PCR Primer, Index 1 (100% over 30bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC670010.5549238904255358No Hit
GCATCCTTAGCTCAGCTGGATAGAGTACTCGGCTGGAATTCTCGGGTGCC522460.43271822180523495No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCTGGAATTCTCGGGTGCCAA497460.4120124155327339No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA494920.4099087056154478RNA PCR Primer, Index 1 (100% over 28bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA486880.4032497183182115No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA468250.3878197514839437No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG426160.35295945604356105No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC376560.31187913639891907No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCTGGAATTCTCGGGTGCCA374730.310363471379772No Hit
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG352960.2923328552776781Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA349430.289409195432001RNA PCR Primer, Index 1 (100% over 28bp)
TTCGAGACCCGCGGGCGCTCCCTGGCCCTTTGGAATTCTCGGGTGCCAAG335570.2779298964345264No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG311020.25759679467493046RNA PCR Primer, Index 1 (100% over 39bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG303660.25150100530830616RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC299230.24783193643681897RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG293200.24283769596389174RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC269770.22343221432530377RNA PCR Primer, Index 1 (100% over 26bp)
GTCAGGATGGCCGAGTGGTCTAAGGCGCCAGATGGAATTCTCGGGTGCCA267820.2218171614360487No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC263310.21808183398448955RNA PCR Primer, Index 1 (100% over 26bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA253110.20963386502530912RNA PCR Primer, Index 1 (100% over 34bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC248750.20602277241138495RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA245110.2030080070181088RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG237670.19684595907141247RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA227410.18834829617717808No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC227380.1883234492096511RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT225210.186526185225198RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTTTTGGAATTCTCGGGTGCCAAGGAACT223920.18545776562153696RNA PCR Primer, Index 1 (100% over 25bp)
TCCTGTACTGAGCTGCCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA212000.17558523719080849RNA PCR Primer, Index 1 (100% over 28bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGATGGAATTCTCGGGTGCCAA209530.17353950353108538No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA201480.16687223391134007RNA PCR Primer, Index 1 (100% over 28bp)
TCTATTTTGTTGGTTTTCGGAACTGAGGTGGAATTCTCGGGTGCCAAGGA198470.16437925483613092RNA PCR Primer, Index 1 (100% over 22bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA198340.16427158464351393RNA PCR Primer, Index 1 (100% over 28bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC196190.16249088530407885RNA PCR Primer, Index 1 (100% over 26bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT194510.16109945512256676RNA PCR Primer, Index 1 (100% over 35bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA191700.15877212249753767RNA PCR Primer, Index 1 (100% over 28bp)
CGAGAATTCTACCACTGAACCACCCATGCTGGAATTCTCGGGTGCCAAGG185830.15391039918475444Illumina Small RNA Adapter 2 (100% over 21bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG181190.15006740154057824RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT181010.14991831973541625RNA PCR Primer, Index 1 (100% over 30bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA176470.14615814531633006RNA PCR Primer, Index 1 (100% over 23bp)
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA175580.14542101861302903RNA PCR Primer, Index 1 (100% over 28bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA171720.14222404212455486No Hit
TTCGAGACCCGCGGGCGCTCCCTGGCCCTTTTGGAATTCTCGGGTGCCAA170580.14127985735852883No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA164470.13621935830552956No Hit
GCGGAAATAGCTCAGTGGTAGAGCACCACCTGGAATTCTCGGGTGCCAAG164280.13606199417785855No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT162240.13437240038602247RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGTGGAATTCTCGGGTGCCAAG158350.13115057693002133No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG157880.1307613077720983No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC154610.12805298831165518RNA PCR Primer, Index 1 (100% over 24bp)
GCGGATGTAGCGCAGCTGGTAGCGCATAACCTGGAATTCTCGGGTGCCAA153040.1267526636777421No Hit
CTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCAA151470.12545233904382908No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT151400.12539436278626606No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG148550.12303390087120095No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC147540.12219738629779191RNA PCR Primer, Index 1 (100% over 26bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG142180.11775806143296769RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA139930.1158945388684426No Hit
TTTGGTCCCCTTCAACCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC134210.11115705039329436RNA PCR Primer, Index 1 (100% over 27bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG133850.11085888678297035RNA PCR Primer, Index 1 (100% over 29bp)
TCTATTTTGTTGGTTTTCGGAACTGAGGCCTGGAATTCTCGGGTGCCAAG133820.11083403981544335No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCTGGAATTCTCGGGTGCCAA133060.11020458330475932No Hit
TCTATTTTGTTGGTTTTCGGAACTGAGGCTGGAATTCTCGGGTGCCAAGG128420.10636158566058314Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT125040.103562160652541RNA PCR Primer, Index 1 (100% over 30bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG123420.10222042440608294RNA PCR Primer, Index 1 (100% over 29bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCTGGAATTCTCGGGTGCCA121510.10063850080686387No Hit
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT121410.10055567758177385RNA PCR Primer, Index 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)