FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1184053
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC15724113.279895410087217RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1027498.677736554022497RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC788186.656627701631599RNA PCR Primer, Index 1 (100% over 27bp)
AGAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGG298082.5174548774421415No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA261832.211303041333454RNA PCR Primer, Index 1 (100% over 28bp)
CAGAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGG218741.8473835208390164No Hit
AGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTG212261.792656240894622No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC170861.4430097301387692RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC158391.3376934985173805RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAAGGAAC150391.270128955376153RNA PCR Primer, Index 1 (100% over 24bp)
TTCAGAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTC142781.2058581837130602No Hit
GAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGT120111.0143971595866064No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA114580.9676931691402327RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC89870.7590031865127659RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG86680.7320618249352014RNA PCR Primer, Index 1 (100% over 29bp)
TCAGAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCG83470.7049515519997839No Hit
TCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCA61590.5201625265085262No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC55980.4727828906307403RNA PCR Primer, Index 1 (100% over 50bp)
CCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAAGGAACTC52970.4473617312738535RNA PCR Primer, Index 1 (100% over 26bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA49930.42168720488018696No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA49130.41493075056606415RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA45620.3852868072628506No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC42740.3609635717320086RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTTTGGAATTCTCGGGTGCCAAGGAACT39100.33022170460275RNA PCR Primer, Index 1 (100% over 25bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC37030.3127393790649574RNA PCR Primer, Index 1 (100% over 27bp)
TTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCC36540.3086010507975572No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC32410.27372085540089847No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA29990.2532825811006771No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA29410.2483841517229381RNA PCR Primer, Index 1 (100% over 28bp)
GTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGC26170.2210205117507409No Hit
AGAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGGATTCTCGGG25320.21384177904198548No Hit
CCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAAG25240.21316613361057318No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCTGGAATTCTCGGGTGCCA24520.2070853247278627No Hit
CCATGCTCTACCGACTGAGCTAGCCGGGCTGGAATTCTCGGGTGCCAAGG24470.20666304633323004Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA24370.20581848954396467RNA PCR Primer, Index 1 (100% over 28bp)
CAGAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGGATTCTCGG19400.163844017117477No Hit
TCCTGTACTGAGCTGCCCCGAGAATGGAATTCTCGGGTGCCAAGGAACTC18500.1562430060140889RNA PCR Primer, Index 1 (100% over 26bp)
AGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGGATTCTCGGGTG18260.15421606971985205No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCTGGAATTCTCGGGTGCCA18090.15278032317810097No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18020.15218913342561524No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG17740.14982437441567228No Hit
GTCGGTAGAGCATGGGTGGTTCAGTGGTAGAATTCTCGGGTGCCAAGGAA17060.14408138824866792RNA PCR Primer, Index 1 (95% over 23bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC16130.13622701010850022RNA PCR Primer, Index 1 (100% over 26bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCTGGAATTCTCGGGTGCC16130.13622701010850022No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA15870.1340311624564103RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG15600.1317508591253939RNA PCR Primer, Index 1 (100% over 29bp)
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCTGGAATTCTCGGGTGCCA15510.13099075801505508No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA13760.11621101420291154No Hit
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA13740.11604210284505846RNA PCR Primer, Index 1 (100% over 28bp)
CCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCAAG13510.11409962222974816No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG12970.1095390155677153No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTGGAATTCTCGGGTGCCAAG12380.10455613051104976No Hit
TTCAGAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGGATTCTC12370.10447167483212322No Hit
CTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAA12220.1032048396482252No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATTCTCGGGTGCCAAGGAAC12130.1024447385378864RNA PCR Primer, Index 1 (95% over 24bp)
TCCTGTACTGAGCTGCCCCGAGAATTGGAATTCTCGGGTGCCAAGGAACT11890.10041780224364957RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)